12/26/2023 0 Comments Snapgene confocal microscopyConsequently, this affects the accuracy of Raman spectrum and Brillouin spectrum detection and reduces the detection accuracy. In addition, when it was used for biological tissue slice sample imaging, the fluorescence signal generated by vertical incidence reduced the signal-to-noise ratio of the sample's Raman spectrum. Secondly, due to the weak Raman and Brillouin scattering spectra and long integration time, confocal spectrum microscope is easily affected by system drift and leads to defocus, thereby affecting the spatial resolution and imaging quality. The existing confocal Raman/Brillouin spectroscopic microscopic imaging technology has a lack of high precision real-time focusing ability, so that the size of the spot focused on the sample changes with the fluctuation of the sample in the scanning process, thus restricting the realization of the theoretical spatial resolution of confocal spectrum microscopy system. Among the existing detection methods for the morphology, mechanics and chemical properties of tissue and cells, confocal Raman spectroscopy can detect the chemical properties of micro-regions of samples without contact and label, and confocal Brillouin spectroscopy can detect the mechanical properties of micro-regions of samples without contact and nondestructive.Ĭombining confocal Raman spectroscopy with Brillouin spectroscopy, simultaneously and in-situ, to detect the three-dimensional morphology, chemical properties and mechanical properties of micro-regions of tissues and even subcellular structures is expected to provide a new means for the detection of multi-dimensional pathological information of tissue and cells.
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